Amperometric microbiosensor as an alternative tool for investigation of D-serine in brain

• Published in: Amino Acids
• Main Author: Zain Z.M.; Ghani S.A.; O'Neill R.D.
• Format: Review
• Language: English
• Published: 2012
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84868151559&doi=10.1007%2fs00726-012-1365-0&partnerID=40&md5=a93aaba6ec285c67ecefeb9441d47ed9

This paper discusses the application of a reagentless, selective microbiosensor as a useful alternative tool for monitoring D-serine in neural samples. The main components of the 125-μm-diameter disk biosensor were D-amino acid oxidase for D-serine sensitivity (linear region slope, 61 ± 7 μA cm-2 mM-1; limit of detection, 20 nM), and poly-phenylenediamine for rejection of electroactive interference. The response time of the biosensor was of the order of 1 s, ideal for 'real-time' monitoring, and detection of systemically administered D-serine in brain extracellular fluid is demonstrated. Exploitation of this probe might resolve queries involving regulation of D-serine in excitotoxicity, and modulation of N-methyl-D-aspartate receptor function by D-serine and glycine in the central nervous system. © 2012 Springer-Verlag.