Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons
Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1-40 sequence (A...
| Published in: | Cellular and Molecular Neurobiology |
|---|---|
| Main Author: | |
| Format: | Article |
| Language: | English |
| Published: |
Springer Science and Business Media, LLC
2013
|
| Online Access: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886438363&doi=10.1007%2fs10571-013-9982-z&partnerID=40&md5=607ecc0836189c6325a831bb686e28df |
| id |
2-s2.0-84886438363 |
|---|---|
| spelling |
2-s2.0-84886438363 Ismail N.; Ismail M.; Mazlan M.; Latiff L.A.; Imam M.U.; Iqbal S.; Azmi N.H.; Ghafar S.A.A.; Chan K.W. Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons 2013 Cellular and Molecular Neurobiology 33 8 10.1007/s10571-013-9982-z https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886438363&doi=10.1007%2fs10571-013-9982-z&partnerID=40&md5=607ecc0836189c6325a831bb686e28df Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1-40 sequence (Aβ1-40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aβ1-40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 μM) and subsequent exposure to 10 μM Aβ1-40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aβ1-40 alone. TQ pretreatment inhibited Aβ1-40- induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aβ1-40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer's disease. © 2013 Springer Science+Business Media New York. Springer Science and Business Media, LLC 02724340 English Article |
| author |
Ismail N.; Ismail M.; Mazlan M.; Latiff L.A.; Imam M.U.; Iqbal S.; Azmi N.H.; Ghafar S.A.A.; Chan K.W. |
| spellingShingle |
Ismail N.; Ismail M.; Mazlan M.; Latiff L.A.; Imam M.U.; Iqbal S.; Azmi N.H.; Ghafar S.A.A.; Chan K.W. Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| author_facet |
Ismail N.; Ismail M.; Mazlan M.; Latiff L.A.; Imam M.U.; Iqbal S.; Azmi N.H.; Ghafar S.A.A.; Chan K.W. |
| author_sort |
Ismail N.; Ismail M.; Mazlan M.; Latiff L.A.; Imam M.U.; Iqbal S.; Azmi N.H.; Ghafar S.A.A.; Chan K.W. |
| title |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_short |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_full |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_fullStr |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_full_unstemmed |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| title_sort |
Thymoquinone prevents β-amyloid neurotoxicity in primary cultured cerebellar granule neurons |
| publishDate |
2013 |
| container_title |
Cellular and Molecular Neurobiology |
| container_volume |
33 |
| container_issue |
8 |
| doi_str_mv |
10.1007/s10571-013-9982-z |
| url |
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84886438363&doi=10.1007%2fs10571-013-9982-z&partnerID=40&md5=607ecc0836189c6325a831bb686e28df |
| description |
Thymoquinone (TQ), a bioactive constituent of Nigella sativa Linn (N. sativa) has demonstrated several neuropharmacological attributes. In the present study, the neuroprotective properties of TQ were investigated by studying its anti-apoptotic potential to diminish β-amyloid peptide 1-40 sequence (Aβ1-40)-induced neuronal cell death in primary cultured cerebellar granule neurons (CGNs). The effects of TQ against Aβ1-40-induced neurotoxicity, morphological damages, DNA condensation, the generation of reactive oxygen species, and caspase-3, -8, and -9 activation were investigated. Pretreatment of CGNs with TQ (0.1 and 1 μM) and subsequent exposure to 10 μM Aβ1-40 protected the CGNs against the neurotoxic effects of the latter. In addition, the CGNs were better preserved with intact cell bodies, extensive neurite networks, a loss of condensed chromatin and less free radical generation than those exposed to Aβ1-40 alone. TQ pretreatment inhibited Aβ1-40- induced apoptosis of CGNs via both extrinsic and intrinsic caspase pathways. Thus, the findings of this study suggest that TQ may prevent neurotoxicity and Aβ1-40-induced apoptosis. TQ is, therefore, worth studying further for its potential to reduce the risks of developing Alzheimer's disease. © 2013 Springer Science+Business Media New York. |
| publisher |
Springer Science and Business Media, LLC |
| issn |
02724340 |
| language |
English |
| format |
Article |
| accesstype |
|
| record_format |
scopus |
| collection |
Scopus |
| _version_ |
1814778510251655168 |