Effects of NAA and BAP, double-layered media, and light distance on in vitro regeneration of Nelumbo nucifera Gaertn. (lotus), an aquatic edible plant

• Published in: Scientific World Journal
• Main Author: Mahmad N.; Mat Taha R.; Othman R.; Saleh A.; Hasbullah N.A.; Elias H.
• Format: Article
• Language: English
• Published: The Scientific World Journal 2014
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84901799355&doi=10.1155%2f2014%2f745148&partnerID=40&md5=47f6bd939401bc5f387cce72a42780d8

In vitro direct regeneration of Nelumbo nucifera Gaertn. was successfully achieved from immature explants (yellow plumule) cultured on a solid MS media supplemented with combinations of 0.5 mg/L BAP and 1.5 mg/L NAA which resulted in 16.00 ± 0.30 number of shoots per explant and exhibited a new characteristic of layered multiple shoots, while normal roots formed on the solid MS basal media. The double-layered media gave the highest number of shoots per explant with a ratio of 2: 1 (liquid to solid) with a mean number of 16.67 ± 0.23 shoots per explant with the formation of primary and secondary roots from immature explants. In the study involving light distance, the tallest shoot (16.67 ± 0.23 mm) obtained from the immature explants was at a light distance of 200 mm from the source of inflorescent light (1000 lux). The plantlets were successfully acclimatized in clay loam soil after 8 months being maintained under in vitro conditions. © 2014 Noraini Mahmad et al.