Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors

Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors

Drug release, diffusion and penetration studies warrant validation of the respective methods prior to their use. Current study aimed to validate an automated diffusion Microette system with six vertical cells for consistency of donor/receptor chambers volumes, temperature in cells, spinning rods’ sp...

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Published in:Latin American Journal of Pharmacy
Main Author: Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
Format: Article
Language:English
Published: Colegio de Farmaceuticos de la Provincia de Buenos Aires 2023
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85162182897&partnerID=40&md5=2e958ea699a3cbecccdb5c8f09e5564b
id 2-s2.0-85162182897
spelling 2-s2.0-85162182897
Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
2023
Latin American Journal of Pharmacy
42
6

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85162182897&partnerID=40&md5=2e958ea699a3cbecccdb5c8f09e5564b
Drug release, diffusion and penetration studies warrant validation of the respective methods prior to their use. Current study aimed to validate an automated diffusion Microette system with six vertical cells for consistency of donor/receptor chambers volumes, temperature in cells, spinning rods’ speeds, and sample volumes during automatic sampling. Evaporation of receptor fluid from collected samples and work-ing conditions for in vitro release for a model drug, α-tocopherol with or without penetration enhancers were also validated using a validated HPLC method. Internal volume variation was found to be 1.86%, with no difference in volumes of 6 receptor chambers at 30 and 60 min and spinning speeds (p > 0.05). Estimated loss of ethanol was < 2, 3 and 5%, respectively in 8, 12 and 24 h, indicating a negligible solvent evaporation within 24 h. Validated automated system showed a good release profile of α-tocopherol (maximum RSD 6.68%). Automated Microette system for α-tocopherol permeation study was suitable. © 2023, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Colegio de Farmaceuticos de la Provincia de Buenos Aires
3262383
English
Article
author Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
spellingShingle Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
author_facet Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
author_sort Ibrahim S.I.; Bukhari N.I.; Kanwal U.; Majeed A.B.A.; Siddique M.I.
title Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
title_short Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
title_full Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
title_fullStr Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
title_full_unstemmed Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
title_sort Transmembrane Diffusion of α-Tocopherol as a Model Drug Across Synthetic Membrane in Automated Vertical Diffusion Cells – Validation of Device and Drug Related Factors
publishDate 2023
container_title Latin American Journal of Pharmacy
container_volume 42
container_issue 6
doi_str_mv
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85162182897&partnerID=40&md5=2e958ea699a3cbecccdb5c8f09e5564b
description Drug release, diffusion and penetration studies warrant validation of the respective methods prior to their use. Current study aimed to validate an automated diffusion Microette system with six vertical cells for consistency of donor/receptor chambers volumes, temperature in cells, spinning rods’ speeds, and sample volumes during automatic sampling. Evaporation of receptor fluid from collected samples and work-ing conditions for in vitro release for a model drug, α-tocopherol with or without penetration enhancers were also validated using a validated HPLC method. Internal volume variation was found to be 1.86%, with no difference in volumes of 6 receptor chambers at 30 and 60 min and spinning speeds (p > 0.05). Estimated loss of ethanol was < 2, 3 and 5%, respectively in 8, 12 and 24 h, indicating a negligible solvent evaporation within 24 h. Validated automated system showed a good release profile of α-tocopherol (maximum RSD 6.68%). Automated Microette system for α-tocopherol permeation study was suitable. © 2023, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
publisher Colegio de Farmaceuticos de la Provincia de Buenos Aires
issn 3262383
language English
format Article
accesstype
record_format scopus
collection Scopus
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