Assessing the stability of uncentrifuged serum and plasma analytes at various post-collection intervals

• Published in: Journal of Laboratory Medicine
• Main Author: Mokhsin A.; Subramaniam P.; Sivaneson S.; Nheu N.; Ramaloo G.; Hanifah A.S.; Mahathevan S.B.; Nadarajah M.; Sampasivam G.; Ismail A.M.; Rahman T.A.
• Format: Article
• Language: English
• Published: Walter de Gruyter GmbH 2024
• Online Access: https://www.scopus.com/inward/record.uri?eid=2-s2.0-85201875745&doi=10.1515%2flabmed-2024-0062&partnerID=40&md5=e5e33b899c58fb614c4d1c0cbee109d8

Objectives: Our study aimed to assess the stability of 26 biochemistry analytes in serum or plasma samples separated from blood samples centrifuged at different time intervals after collection, simulating sample transport via despatch delivery systems. Methods: Blood from forty-one volunteers were collected using five serum separator tubes (SST) and five fluoride oxalate tubes (FOT) for each volunteer following written informed consent. Each of the five tubes in both groups of SST and FOT was centrifuged at one of the time intervals: 0.5 h, 4 h, 8 h, 12 and 24 h after collection. These samples were left standing prior to centrifugation at room temperature. We calculated the percentage difference for each analyte between the 0.5 h and other time intervals to assess analyte stability. The percentage difference was compared to the desirable specification for bias and reference change value (RCV). Results: Mean concentration of serum potassium showed a significant increase in the percentage RCV after 8 h, while CKMB showed an increase after 12 h of delayed centrifugation compared to the baseline (0.5 h). There were no significant percentage RCV for the other analytes at all timelines. Conclusions: Serum potassium and CKMB were stable up to 8 and 12 h of delayed centrifugation respectively whilst all other analytes appear stable up to 24 h, suggesting that sample transport delay of up to 8 h, with the condition that room temperature is maintained, may not have a significant impact on accuracy of the biochemistry/immunochemistry test results. © 2024 the author(s), published by De Gruyter.