| Summary: | This study assessed the effects of 6-Benzylaminopurine and wood vinegar on in vitro shoot multiplication, and as elicitors in enhancing the phytochemical content of Clinacanthus nutans extract. A nodal explant of C. nutans was cultured in vitro with single or combination treatments in MS medium supplemented with 6-Benzylaminopurine (BAP) or wood vinegar (WV). The growth performance of regenerated shoots was documented after eight weeks of culture. The total phenolic content, total flavonoid content, and antioxidant activities of the leaf extracts were also studied. The results demonstrated that all single treatments with BAP successfully regenerated and multiplied shoots and leaves. While in combination treatments, the data revealed that 2 mg/L BAP with 1% WV (B2WV1) medium treatment led to the highest number of shoots and leaves per explant and the highest total phenolic content and antioxidant activities in the leaf extract. This concludes that combining wood vinegar and BAP treatments in the culture medium caused significant shoot and leaf growth and enhanced the production of leaf’s secondary metabolites. These findings highlight the potential of 6-Benzylaminopurine and wood vinegar as elicitors to enhance the production of secondary metabolites in Clinacanthus nutans, providing valuable insights for further research in plant biotechnology. © 2024, Malaysian Society of Analytical Sciences. All rights reserved.
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