| Summary: | This study explored the anti-inflammatory potential of halogenated chalcone derivatives using in-silico, in-vitro and in-vivo models. The synthesized compounds were assessed for their ability to inhibit reactive oxygen species (ROS) production and their interaction with the cyclooxygenase-1 (COX-1) enzyme, a target of nonsteroidal anti-inflammatory drugs (NSAIDs). In-vitro assays demonstrated that these chalcone derivatives effectively reduced ROS levels, suggesting robust anti-inflammatory activity. This was further supported by in-vivo findings from the rat paw edema model, where treated groups exhibited a significant reduction in paw thickness compared to controls. Histopathological analysis confirmed a decrease in leukocyte infiltration in the treated groups, aligning with the observed anti-inflammatory effects. Molecular docking studies indicated strong binding affinities of the chalcone derivatives to the COX-1 enzyme, implying a similar mechanism of action to established NSAIDs like ibuprofen. Additionally, in-silico ADME (absorption, distribution, metabolism and excretion) profiling revealed favorable pharmacokinetic and drug-like properties, highlighting the therapeutic potential of these compounds. Overall, halogenated chalcone derivatives show promising anti-inflammatory properties through ROS inhibition and COX-1 binding, warranting further research to optimize their efficacy and validate their potential as new anti-inflammatory agents. © 2024 Elsevier B.V.
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