Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]

Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]

Thermostable cellulase from fungi has high potential for industrial application. In this study, wild -type of fungal were isolate from different sources such as hot spring water, sea water, soft wood, rice straw and cow dung. The isolates were characterized by cultural and morphological observation....

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发表在:Malaysian Journal of Analytical Sciences
主要作者: 2-s2.0-84939474459
格式: 文件
语言:English
出版: Malaysian Society of Analytical Sciences 2015
在线阅读:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939474459&partnerID=40&md5=b1f80e2f4783e09ccfa181b939a8ad2f
id Jamroo N.A.; Umor N.A.; Kamsani
spelling Jamroo N.A.; Umor N.A.; Kamsani
2-s2.0-84939474459
Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
2015
Malaysian Journal of Analytical Sciences
19
4

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939474459&partnerID=40&md5=b1f80e2f4783e09ccfa181b939a8ad2f
Thermostable cellulase from fungi has high potential for industrial application. In this study, wild -type of fungal were isolate from different sources such as hot spring water, sea water, soft wood, rice straw and cow dung. The isolates were characterized by cultural and morphological observation. Based on morphological characteristics, the genera of all fungal cultures were identified namely Aspergillus fumigatus. The screening for thermostable cellulase were done using 2% carboxymethyl cellulose and congo red as an indicator at temperature 30˚C, 37˚C, 45˚C and 50˚C respectively. Out of 26 fungal isolates, only eight isolates were selected for further screening and showed the abilities to secrete cellulases by forming distinct halo zones on selective agar plate. The maximum halo zone ranging from 32mm to 35mm were obtained after 72 hour incubation at 50˚C by H2, SW1 and C1 isolates. As contrary other isolates showed halo zone range from 22 mm to 29 mm at same temperature. All the isolates showed the abilities to secrete cellulase enzyme at other temperature but lower when compared to 50˚C referred to the halo zone obtained. The SW1 isolates showed highest celluloytic index which was 2.93measured at 37 ˚C and 2.67 at 50˚C respectively. © 2015, Malaysian Society of Analytical Sciences. All rights reserved.
Malaysian Society of Analytical Sciences
13942506
English
Article
author 2-s2.0-84939474459
spellingShingle 2-s2.0-84939474459
Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
author_facet 2-s2.0-84939474459
author_sort 2-s2.0-84939474459
title Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
title_short Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
title_full Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
title_fullStr Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
title_full_unstemmed Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
title_sort Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature; [Pengasingan dan Saringan Enzim Selulosa Tahan Haba dari Kulat pada Suhu yang Berbeza]
publishDate 2015
container_title Malaysian Journal of Analytical Sciences
container_volume 19
container_issue 4
doi_str_mv
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939474459&partnerID=40&md5=b1f80e2f4783e09ccfa181b939a8ad2f
description Thermostable cellulase from fungi has high potential for industrial application. In this study, wild -type of fungal were isolate from different sources such as hot spring water, sea water, soft wood, rice straw and cow dung. The isolates were characterized by cultural and morphological observation. Based on morphological characteristics, the genera of all fungal cultures were identified namely Aspergillus fumigatus. The screening for thermostable cellulase were done using 2% carboxymethyl cellulose and congo red as an indicator at temperature 30˚C, 37˚C, 45˚C and 50˚C respectively. Out of 26 fungal isolates, only eight isolates were selected for further screening and showed the abilities to secrete cellulases by forming distinct halo zones on selective agar plate. The maximum halo zone ranging from 32mm to 35mm were obtained after 72 hour incubation at 50˚C by H2, SW1 and C1 isolates. As contrary other isolates showed halo zone range from 22 mm to 29 mm at same temperature. All the isolates showed the abilities to secrete cellulase enzyme at other temperature but lower when compared to 50˚C referred to the halo zone obtained. The SW1 isolates showed highest celluloytic index which was 2.93measured at 37 ˚C and 2.67 at 50˚C respectively. © 2015, Malaysian Society of Analytical Sciences. All rights reserved.
publisher Malaysian Society of Analytical Sciences
issn 13942506
language English
format Article
accesstype
record_format scopus
collection Scopus
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